Description
"Yu-Jung Su1 (蘇昱融), You-Hsuan Liu1,2 (劉又萱), Bor-Lin Huang1 (黃柏霖), Karen G. Rosal1 (羅凱倫) and Keng-Hui Lin1 (林耿慧)
- Institute of Physics, Academia, Sinica, Taipei, Taiwan
- Genome and Systems Biology Degree Program, National Taiwan University, Taipei, Taiwan
Abstract:
Cells grown in 3D environment often exhibit different morphological and epigenic response from cells grown on traditional 2D culture. Previously, our lab demonstrated that spherical microwells serve as good approximation for 3D culture. However, the throughput of microwells in earlier study was low, only 10 ´ 10 microwells were generated for each chip. In this study, we created O(100 ´ 100) microwells on a 22´22 mm2 coverglass and cultured human mesenchymal stem cells (hMSCs) in the large array. This new chip generates enough cells for flow cytometry and RNA-seq analysis. We found hMSCs in small microwells do no proceed normal cell cycle and higher cytoplasmic retention of yes associated protein (YAP) in cells in small microwells (60 mm) compared with cells in 100-mm microwells. We also found that hMSCs prefer adipogenic differentiation in spherical microwells. Transcriptomic analysis show that differentiation-related genes are up-regulated and chromosome-related genes are down-regulated. The analysis agrees with our phenotypic observation."
