Description
Nanodiamonds (NDs) are promising material for various biological purposes. In this study, detonation method produced Nanodiamonds with an average size of 5 nm had been certificated the antibacterial properties. We combined NDs with 3D printing material Polycaprolactone (PCL) to create an antibacterial composite. Raman spectroscopy played a pivotal role in characterizing ND-PCL, verifying the combination of ND and PCL was successful. Furthermore, Raman mapping images of ND-PCL were obtained to confirm the uniform distribution of ND throughout the composite. After sample preparation, we subsequently investigated the antibacterial effect of ND-PCL against gram-negative bacteria Escherichia coli (E. coli). UV-visible spectroscopy was employed to observe the interaction between ND-PCL and E. coli after 24 hrs incubation. Bacterial viability was monitored by measuring optical densities of E. coli at 600 nm in nutritious liquid Luria–Bertani medium for 24 hrs incubation. The outcome demonstrated the biocompatibility of PCL, and the antibacterial effect was attributed to ND. By comparing the results between pure ND and ND-PCL, we certified the combination didn’t reduce the antibacterial effect of ND. This study elucidated an application for ND in forming an antibacterial composite with 3D-printing materials, we hoped the continued research and development can explore potential of ND composite and utilize in medicine application.
