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Description
In this work, we address the participation of primary and secondary “Carotenoids” in the life activity of the regenerating anterior region of A. viride. A. viride is tasked with building entire body segments out of their single starting cell at their amputated region and undergoing epimorphic regeneration, therefore, these annelids are the most suitable for the study of regeneration. In regenerative organisms, regeneration arises with the help of repatterning co-existing tissues after a wound or trauma has occurred. Understanding these mechanisms is crucial as critical metabolic functions are involved during the process of regeneration. A time-dependent study with Resonance Raman spectroscopy at 532 nm wavelength excitation has been acquired from the regenerated site of freshwater A. viride. The Raman spectra at different hours post-amputation (hpa) of A. viride were recorded to elucidate the molecular composition in the regenerating newly formed stem cells. We evaluated the interplay of reactive oxygen species and carotenoids and their vital role in the anterior regeneration of A. viride. In vivo intracellular imaging; of blastema bud was made possible by applying Two-Photon Fluorescence Lifetime Imaging in combination with the phasor analysis. These findings indicate that the Raman signature of carotenoids can be used to detect the process of newly derived stem cells in the study of regeneration.
