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Shilpa Kumari (PhD student)Poster
Amyloid beta (Aβ) protein accumulation is a major hallmark of Alzheimer’s disease (AD), primarily occurring in the hippocampal region of the brain and associated with impaired microglial clearance mechanisms, oxidative stress, and chronic neuroinflammation. Growing evidence suggests that accumulated Aβ and oxidative stress affect bioenergetic pathways by inducing a metabolic shift in microglia...
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Poster
Directional reversals are a mechanism where bacteria undergo a drastic change in their moving directions by switching to the opposite way. Such behavior is seen in microorganisms like Myxococcus xanthus, and is essential for them to swarm and aggregate. In this study, we investigate the role of directional reversals in bacterial pattern formation. We propose and analyze a minimal model...
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Yu-Sheng Liu (Department of Optoelectronic Physics, Chinese Culture University, Taipei, Taiwan)Poster
Traditionally, the analysis of biological images has mostly relied on manual operations. In the context of this study, the experimental workflow involves at least the following steps: selecting samples, performing feature extraction, image segmentation to obtain regions of interest (ROI), generating plots and statistics, and conducting image visualization. However, with the rapid advancement...
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Josephine Chia-Ling Liang (Institute of Physics, Academia Sinica; Graduate Institute of Applied Physics, National Taiwan University)Poster
Microridges are laterally elongated, actin-rich protrusions often found on the apical surface of superficial epithelial cells of zebrafish, where they form evenly spaced, maze-like patterns that continuously remodel through fission and fusion. These conserved structures play key roles in mucus retention and in organizing surface molecules. The studies on microridges are relatively low compared...
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YU-WEI CHIANG (Graduate school of Advanced Technology, National Taiwan University)Poster
Cancer invasion, an indicator of deadly metastasis, is governed by complex tumor microenvironment (TME) interactions, including ECM stiffness. Understanding invasion dynamics is essential for developing effective therapies. In this study, we investigated how ECM stiffness and cellular composition affect cancer invasion using 3D spheroid models. Our results demonstrate that spheroid invasion...
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Invited Speaker
Recent advances in genetics, biochemistry, cell biology, and biophysics have contributed to our understanding that the genome is organized hierarchically, with higher order macromolecular assemblies spanning spatial and temporal orders of magnitude. Beyond being wrapped around histones as irregular groups of “beads on a string”, chromatin is organized into topologically associated domains,...
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Prof. Yonggun Jun (Department of Physics, National Central University)
Kinesin motors are essential for intracellular organelle transport. While extensive studies have focused on understanding their collective motion, the reasons for the wide distribution of cargo speeds in cells remain poorly understood. In this study, we examine the relationship between motor numbers, cellular crowding, and cargo velocity during cargo transport in vitro. Our findings indicate...
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Zhe-Yuan Zhang (Institute of Physics, Academia Sinica；Graduate Institute of Applied Physics, National Taiwan University, Taipei, Taiwan)Poster
Substrate geometry affects collective cell migration. Recently, curvotaxis is identified where cells exhibit different preference toward curvature. To further explore the curvotaxis, we fabricated pneumatic-driven microdomes to create the substrate curvature from flat to positively curved surface and observed a novel migratory behavior: epithelial (MDCK) cells exhibit a strong, centripetal...
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Chromatin, in which genetic information in a eukaryotic cell is stored, is a long polymer-like composite of DNA and proteins densely packed in the nucleus. It is now recognized that physical spacing of chromatin is critical in regulating bio-chemical and transcriptional abilities of genes, and proper functionality depends on the nonrandom organization of chromatin. Meanwhile, in a living cell,...
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Chia-chun Yeh (National Taiwan University)Poster
Introduction
Cells in vivo encounter geometrically complex microenvironments where curvature at the cellular scale can regulate cell morphology and migration [1]. Reduced ECM curvature near solid tumors, for instance, correlates with increased metastatic rate [2]. These observations have motivated the concept of curvotaxis, describing changes in cell movement due to the curvature cues...
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Prof. Min-Yeh Tsai (National Chung Cheng University)Invited Speaker
Amyloid-β (Aβ) aggregation provides a prototypical example of geometry-regulated self-assembly in biological systems. While amyloid growth is often described in terms of chemical kinetics or tip-growth models, how single-molecule diffusion on fibril surfaces is shaped by physical constraints remains incompletely understood. Here, we use coarse-grained molecular dynamics simulations to...
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Jheng-Syong Wu (Academia Sinica)Poster
RNA polymerase III (Pol III) synthesizes short RNAs with exceptional efficiency. Unlike Pol II, Pol III retains TFIIE- and TFIIF-like sub-complexes that give it unique capabilities during different stages in transcription. Yet the role of the tandem-winged-helix (tWH) domain of Rpc34 during elongation has remained unresolved, in part because its density is consistently missing from cryo-EM...
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Neil Adrian Ondevilla (Institute of Physics, Academia Sinica)Poster
How bacterial cells maintain energetic balance under complete nutrient deprivation remains largely unknown. In this study, the single-cell energy dynamics of Escherichia coli exposed to extreme starvation, a condition in which no external nutrients are available, was investigated. Using QUEEN-2m, a genetically encoded fluorescence ATP sensor, intracellular ATP levels in individual cells were...
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Pragya Pragya (Institute of Physics, Academia Sinica)Poster
Three-dimensional (3D) spheroid models are a cornerstone of advanced 3D cell culture, offering physiologically relevant microenvironments compared to traditional 2D systems. Controlling spheroid size is essential because drug penetration, hypoxia, and necrosis are strongly size-dependent. Conventional methods often yield variable spheroids, limiting their application potentials to be...
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Yi Ting Lin (Dept. of Chemistry, National Chung Cheng University, Taiwan)Poster
ABSTRACT: Amyloid β (Aβ) aggregation on neuronal membranes is a key event in the pathogenesis of Alzheimer’s disease. Among Aβ isoforms, Aβ42 is particularly pathogenic due to its rapid aggregation and high cytotoxicity. Ganglioside GM1, a glycosphingolipid enriched in neuronal membranes, can form nanoclusters that strongly bind Aβ and induce membrane-associated aggregation. Upon binding to...
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Hsiang-Yu Chen (Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan；Department of Physics, National Taiwan University, Taipei, Taiwan)Poster
Correlation-based analysis has long served as a powerful framework for extracting dynamic and structural information beyond conventional imaging limits. Fluorescence-based super-resolution optical fluctuation imaging (SOFI) leverages temporal intensity fluctuations from the stochastic blinking of fluorophores, achieving resolution enhancement through high-order cumulant analysis. However,...
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Pramath Jain (GIBMTE, Taipei medical university)Poster
Extracellular vesicles (EVs) have become recognized as promising natural nanocarriers for targeted drug delivery owing to their biocompatibility and intrinsic cell-specific interactions. This study describes the development of an EV-based nanocarrier system for cancer therapy using ultrasmall gold nanoclusters (1–2 nm) coated with a pH-sensitive PDPH linker connected to curcumin...
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Ryo SuzukiInvited Speaker
The freshwater cnidarian Hydra is well known for its almost unlimited regenerative capacity. Even when cut into several pieces, each fragment can regenerate into a complete animal within approximately one week. Owing to its exceptional simplicity, Hydra allows simultaneous tracking of regeneration associated gene expression and tissue deformation throughout the regeneration process, from...
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Po-Chun Yang (Chemistry, National Taiwan University)Poster
DMC1 recombinase is essential for meiotic homologous recombination. Recombinases, such as RAD51, can assemble on DNA to form extended nucleoprotein filaments to function in homology search and strand exchange. Surprisingly, our recent single-molecule measurements show that human DMC1 can form condensed, compact filaments on double-stranded DNA . These condensed filaments differ markedly from...
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Cheng-han Yu (The University of Hong Kong)
Integrin activation dynamically orchestrates adhesion formation and cell motility within the heterogeneous extracellular space. However, mechanosensitive regulation of integrin β6 remains poorly understood. Using a traction‑free RGD membrane, integrin β6 colocalizes and nucleates micrometer‑scale clusters of viscous RGD ligands by 15 minutes and subsequently dissipates by 60 minutes. Swapping...
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Alan Givré (Academia Sinica)Poster
We start with a model where the bacteria are trying to maximize the long-term access to a chemoattractant and,
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using results in gambling thermodynamics, we get a boundary for the drift speed that is linear in the information
rate. This boundary becomes relevant at extremely low chemoattractant concentrations. -
Shannon Yan (Stanford University)
How do cells physically divide? Successful mitosis requires coordinated cortical dynamics that mechanically drive cell morphological changes in space and time. Yet, the field lacks a unified, quantitative description of such physical processes due to limitations in existing methodologies. Here, we present live-cell membrane tension measurements during mitosis in mouse embryonic stem cells....
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Prof. Jean-Cheng Kuo (Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University)Invited Speaker
Sarcopenia, a prevalent muscle disease characterized by muscle mass and strength reduction, is associated with impaired skeletal muscle regeneration. However, the influence of the biomechanical properties of sarcopenic skeletal muscle on the efficiency of the myogenic program remains unclear. Herein, we established a mouse model of sarcopenia and observed a reduction in stiffness within the...
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Ms You-Hsuan Liu (Department of Physiology, Development and Neuroscience, University of Cambridge)Poster
During development, cells migrate throughout the body along morphogenesis, often encountering mechanical stress that deforms their nuclei, potentially influencing gene expression. In zebrafish embryos, trunk neural crest cells (TNCs) are an embryonic cell type that serves as an ideal model for studying in vivo cell squeezing. They originate from a progenitor region with large interstitial...
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KengHui Lin (Institute of Physics, Academia Sinica)Invited Speaker
This talk will cover two parts of my research. The first part is on my lab's long-term efforts to use spherical pores as 3D cell cultures. Microwell arrays have emerged as three-dimensional substrates for cell culture due to their simplicity of fabrication and promise for high-throughput applications such as 3D cell-based assays for drug screening. To date, most microwells have had cylindrical...
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Dr Nikhil Bhatia (Academia Sinica)Poster
The bacterial flagellum represents one of the most intricate macromolecular assemblies mediating bacterial motility, comprising approximately two dozen distinct proteins. Its architecture includes a rotary motor, a universal joint, and a helical external filament, the latter primarily constructed from flagellin subunits. The majority of flagellar protein subunits are translocated to the...
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Liu Cheng-Han (National Chung Cheng University)Poster
This study investigates the mechanisms underlying the interaction between a single-stranded DNA aptamer and vancomycin. Experimental observations indicate that the aptamer exhibits high selectivity in binding to vancomycin, inducing conformational changes that lead to fluorescence quenching. However, the precise mechanisms remain unclear. To clarify these mechanisms, molecular dynamics (MD)...
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Ja Yil Lee (Department of Biological Sciences, Ulsan National Institute of Science and Technology)Invited Speaker
Chromatin dynamically alters according to cell cycles and DNA metabolic events. One key factor for the chromatin dynamics is histone chaperones that serve nucleosome assembly and disassembly and play important roles in regulating gene expression as well as for maintaining epigenetic information. It has been reported that nucleosome arrangements are modulated by bromodomain-containing AAA+...
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Mr PRADEEP KUMAR (Taipei medical university)Poster
Abstract. Alzheimer’s disease is a neurological disorder characterised by the abnormal accumulation of the proteins including amyloid beta and neurofibrillary tangles, and is closely associated with gradual increase in mitochondrial viscosity. Amyloid-β protein produced during the amyloid cascade interacts with metal ions, disrupts electron transport chain (ETC) complex assembly, and alters...
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Prof. Tony Yang (National Taiwan University)
Direct stochastic optical reconstruction microscopy (dSTORM) overcomes the diffraction limit of light, enabling nanoscale visualization (~10–20 nm) of subcellular structures. Yet, achieving true ultrastructural resolution with dSTORM alone remains challenging. Recent advances combining dSTORM with expansion microscopy (ExM)—which physically enlarges specimens via a swellable polymer...
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Dr Pin-Wu LiuPoster
Learning and memory rely on long-lasting yet adaptable changes in synaptic function. These changes require precise nanoscale organization of post-synaptic proteins, but the mechanisms that establish and regulate this organization remain unclear. Here we show that the multivalent dodecameric kinase CaMKII undergoes Ca²⁺-dependent liquid–liquid phase separation (LLPS) with the NMDA receptor...
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Poster
Structural colour in nature provides a powerful platform for studying how optical function emerges from constrained material systems.
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In this work, I investigate natural photonic crystals as coupled problems of architecture, composition, and mechanics.
Using cuticular photonic structures as model systems, I combine three-dimensional electron tomography, refractive-index–sensitive imaging,... -
Min Ju Shon (POSTECH)Invited Speaker
Tau is classically known for its microtubule binding and association with neurodegenerative disease, but its ability to form condensates suggests a broader role in cellular organization. In this talk, I will introduce how single-molecule and cell-based assays reveal that tau binds strongly to naked DNA and nucleosomes. Such interactions drive co-condensation into mobile, liquid-like clusters...
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Claire Tagasa (Institute of Physics, Academia Sinica)Poster
Micropatterning has been shown a powerful technique to decouple the effects between the specific cell shape, area, aspect ratio, and adhesion distributions on 2D. However, to pattern cells in 3D is still quite limited, especially, at the subcellular scale. Here, we use direct writing method by the commercial PRIMO system to micropattern extracellular matrix (ECM) proteins, such as fibronectin,...
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Ms Shao-Jung Lin (Taipei Medical University, School of Health Care Administration, bachelor), Mr Hung-Yi Liu (Taipei Medical University, institute of optobimechatronics department, research assistant)Poster
Sarcopenia is a gradual decrease in muscle mass usually closely related to aging. Current treatments include strength training, dietary management, and various medications; however, these methods have limitations in efficacy, safety, and feasibility. Therefore, novel and more effective treatment strategies remain a critical area of exploration. This study investigates the application of...
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Chiwei Lee (Institute of Physics, Academia Sinica)Poster
Mechanical forces shape cells and also influences gene expressions. Precise morphological measurement gives us the insight on the mechanical forces experienced by the cells. Current state-of-art deep-learning segmentation methods, such as Cellpose, gives reliable segmented images, whose boundary is at the pixel resolution. The pixel-size resolution can give rise to more than 10% errors in...
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Dr Uttam Kumar (Institute of Physics, Academia Sinica, Taiwan)Poster
Bacteria use quorum sensing (QS), a process based on the production and detection of autoinducers, to coordinate gene expression in response to environmental cues and population density [1]. Although the molecular mechanisms of QS are well understood, its evolutionary role in natural environments remains unclear. In fluctuating conditions, autoinducer signals can become unreliable. Recent...
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Bhuvan Lokesh Marenahalli (Ph.D. Student at Taipei medical university, Taiwan)Poster
Abstract. In recent years, the significance of Promethazine hydrochloride (PMZ) drugs has grown exponentially in both human and veterinary healthcare systems. Improperly getting rid of residues can contaminate environmental samples and food products. Therefore, a rapid detection and efficient treatment of PMZ is highly desired. In this study, the hydrothermal synthesis of calcium molybdate...
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HSIUFANG Fan (National Sun Yat-sen University)Invited Speaker
Mitochondrial DNA (mtDNA) integrity is essential for proper cellular function and relies on the coordinated activities of several mitochondrial proteins. In this study, we employed two single-molecule fluorescence-based approaches—single-molecule Förster resonance energy transfer (smFRET) and nuclease-induced stepwise photodropping (NISP)—to investigate the mechanistic roles of the...
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Tun Liang Chang (Department of Chemistry, National Taiwan University)Poster
Programmed double-strand breaks (DSBs) are essential in meiosis to generate genetic diversity through homologous recombination (HR). A critical step in HR involves the assembly of Dmc1 recombinases on the single-stranded DNA (ssDNA) bound by the abundant Replication Protein A (RPA) proteins. This rate-limited process is regulated by accessory proteins such as Mei5-Sae3 in budding yeast...
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Wei-Hsiang Lin (Institute of Molecular Biology, Academia Sinica)
ATP (adenosine triphosphate) is the common energy carrier for all living systems and is essential for cell metabolism and RNA synthesis. It is constantly converted between ATP and ADP (adenosine diphosphate) form and hence the ATP:ADP is an important indicator for energy balance of the cells. Using microfluidic cell culture with an ATP fluorescence biosensor, we measured the long-term ATP...
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Chia-Hsin Wu (Institute of Physics, Academia Sinica)Poster
In addition to biochemical factors, physical factors of the cellular microenvironment have been widely recognized as important in determining cell behaviors. For example, substrate stiffness increases cell spread area, proliferation, and osteogenic differentiation in 2D cell culture. In contrast, cells may exhibit different responses to substrate stiffness in three-dimensional (3D) cell...
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7. The Mechanical Signature of Disease: Equilibrium and Dynamics from Single Molecules to Live CellsProf. Ching-Hwa Kiang (Rice University)Invited Speaker
Single-molecule and single-cell force measurements provide essential quantitative insights into the thermodynamics and mechanics of complex biological systems. We utilize atomic force microscopy (AFM) to analyze materials across a range of length scales, from individual biopolymers to live cells. At the molecular level, we explore the force response, equilibrium states, and dynamic properties...
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Chun-Yi Ma (Chemistry, National Taiwan University)Poster
In eukaryotes, linear chromosomes face the "end-replication problem" due to the inherent limitations of the degradation of RNA primers involved in lagging-strand synthesis. To maintain genomic integrity and prevent gradual loss of genetic information in the terminal, organisms utilize telomeres—specialized TG-rich 3’ overhangs DNA structure (G-strand). In budding yeast, telomere maintenance...
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Chen-Wei Hsu (Institute of Physics, Academia Sinica)Poster
Epithelial cells display apical membrane protrusions with diverse morphologies and organizations, including microvilli and microridges. Microvilli have been well-reported, which protrude from dots. In contrast, microridges are less studied, which protrude from lines. The lines are often arranged in maze-like patterns. Microridges are found on the superficial epithelial cells (SEC) of...
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Po-Kai Wang (Academia sinica)Poster
The tight junction outlines the apicolateral border of epithelial cells like a belt, sealing the paracellular space when cells form epithelial sheets. The permeability and morphology of tight junction are regulated by actomyosin contractility, which has been conventionally thought from the purse-string-like circumferential actomyosin belt along tight junction. Spatially, the tight junction is...
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Ms Thi Tram Doan (Institute of physics academia sinica)Poster
Neutrophils, the most abundant leukocytes in the human body, serve as the first line of defense against infection and injury. They locate and eliminate pathogens through a “search-and-run” strategy, consisting of an initial random search phase followed by a directed migration toward chemical cues. While the directed phase has been extensively studied, the mechanisms underlying the random...
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You-Ren Chen (Chemistry, National Taiwan University)Poster
DNA lesions ahead of replication forks can stall DNA replication. To maintain DNA fork stability and activate downstream DNA repair pathways, fork-remodeling enzymes process stalled forks into reversed forks. In humans, SMARCAL1 is thought to function at the early stage of fork reversal. Upon fork stalling, replication protein A (RPA), a high-affinity single-stranded DNA (ssDNA)-binding...
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Navaneethan Radhakrishnan (National Center for Theoretical Sciences, Physics Division, National Taiwan University, Taipei)Poster
Identifying functionally relevant allosteric sites is a non-trivial problem, as such sites are often not evident from static structures and depend on protein dynamics. From an information-theoretic perspective, this problem can be framed as identifying regions that share mutual information relevant to fluctuations at a designated active site. Effective allosteric regulation involves selective...
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Koushik Goswami (NCTS Physics, NTU)Poster
Extracting useful work from environmental fluctuations—or, equivalently, minimizing the energetic cost of driving a system—has long been a central goal in physics and engineering. This pursuit is inspired by biological systems, such as motor proteins, which achieve directed motion in intrinsically noisy environments. In synthetic settings, however, work extraction typically requires carefully...
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Mahmoud Ahmed (Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 40227, Taiwan)Poster
Humic substances play critical roles in environmental remediation and soil fertility, yet natural humification requires decades to centuries. Here, we report a synergistic approach combining microbial fermentation with manganese dioxide (MnO₂) catalysis to produce mature humic-like substances (HLS) within 15 days. This work represents the first systematic demonstration of yeast-mineral synergy...
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