Speaker
Description
Microridges are laterally elongated, actin-rich protrusions often found on the apical surface of superficial epithelial cells of zebrafish, where they form evenly spaced, maze-like patterns that continuously remodel through fission and fusion. These conserved structures play key roles in mucus retention and in organizing surface molecules. The studies on microridges are relatively low compared with another kind of apical protrusions, microvilli. We developed an image analysis pipeline to quantify the characteristics of microridge such as its length distribution, average spacing, domain size and so on and also geometric characteristics of cells. Together, these approaches allow us to analyze microridge dynamics under perturbation, providing insight into how epithelial surfaces build microridges.
