Speaker
Description
Three-dimensional (3D) spheroid models are a cornerstone of advanced 3D cell culture, offering physiologically relevant microenvironments compared to traditional 2D systems. Controlling spheroid size is essential because drug penetration, hypoxia, and necrosis are strongly size-dependent. Conventional methods often yield variable spheroids, limiting their application potentials to be quantitative. Our spherical microwell platforms offer a reliable method for generating highly uniform spheroids by confining cells in defined geometrical wells. Here, I will describe the fabrication of spherical microwell arrays with small openings which are suited for spheroid culture of uniform size. I will use this platform for high-throughput image analysis to screen for drug efficacy.
