Speakers
Description
Sarcopenia is a gradual decrease in muscle mass usually closely related to aging. Current treatments include strength training, dietary management, and various medications; however, these methods have limitations in efficacy, safety, and feasibility. Therefore, novel and more effective treatment strategies remain a critical area of exploration. This study investigates the application of phototherapy in the treatment of sarcopenia.
In our studies we found that the infrared light from photomodulation (PBM) at a wavelength of 830 nm and the fluence of 5 J/cm2 significantly enhances the quantity of extracellular vesicles (EV) produced by adipose-derived stem cells (ADSC). Our study focuses on the and function of these PBM-treated ADSC-EVs, which were isolated via ultracentrifugation, then subsequently applied to mouse myoblasts (C2C12) to evaluate their effects on cell proliferation and migration.
EV characterization was performed using Nanoparticle Tracking Analysis (NTA) and Dynamic Light Scattering (DLS) to assess size distribution and zeta potential, while the "Leprechaun" system was utilized to verify specific CD markers. Functional assays, including CCK-8 and wound-healing migration assays on C2C12, were conducted to measure cell viability and motility.
These results suggest that PBM preconditioning optimizes the therapeutic profile of stem cell-derived EVs. This work provides a foundation for developing advanced photobiomodulation-based therapies to promote muscle repair and address the clinical challenges of sarcopenia.
