The 4th Physical Biology and Biological Physics Symposium - The Biological Physics Across Scales From Single Molecules to Organisms

Session

Single molecule assay to precisely investigate mitochondrial DNA integrity maintenance mechanism-mediated by TWINKLE, mySSB and MGME1

Session V - 2 Chair: Yu-chun Yolanda Lin

17 Jan 2026, 09:30

Conveners

Single molecule assay to precisely investigate mitochondrial DNA integrity maintenance mechanism-mediated by TWINKLE, mySSB and MGME1

• HSIUFANG Fan (National Sun Yat-sen University)

9. Single molecule assay to precisely investigate mitochondrial DNA integrity maintenance mechanism-mediated by TWINKLE, mySSB and MGME1

HSIUFANG Fan (National Sun Yat-sen University)

Invited Speaker

Mitochondrial DNA (mtDNA) integrity is essential for proper cellular function and relies on the coordinated activities of several mitochondrial proteins. In this study, we employed two single-molecule fluorescence-based approaches—single-molecule Förster resonance energy transfer (smFRET) and nuclease-induced stepwise photodropping (NISP)—to investigate the mechanistic roles of the...